Hotspot

ycf1

SSC · 123387-128930 · 5544 bp

Back to Yamazakia

Pi 0.0195
MarkerSeek score 48.80
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5544
variable_sites
108
pi
0.0195
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CGTTTCATCGATTTTGTTCGGA AGGAACTTTACAAACGGAAGA 40.9 38.1 57.9 55.1 166 bp 1.0 43.9
ycf1_p2 CGTTTCATCGATTTTGTTCGGA AGGAACTTTACAAACGGAAGAA 40.9 36.4 57.9 55.9 166 bp 1.0 43.9
ycf1_p3 TCGTTTCATCGATTTTGTTCGG AGGAACTTTACAAACGGAAGA 40.9 38.1 57.9 55.1 167 bp 1.0 43.8
ycf1_p4 TCGTTTCATCGATTTTGTTCGG AGGAACTTTACAAACGGAAGAA 40.9 36.4 57.9 55.9 167 bp 1.0 43.8
ycf1_p5 CGTTTCATCGATTTTGTTCGGA ACCATCACCCATTCTTACTAAGA 40.9 39.1 57.9 56.7 196 bp 1.0 43.3