Hotspot

rpl16-rps3

LSC · 83832-83999 · 168 bp

Back to Yamazakia

Pi 0.0482

MarkerSeek score 77.30

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 168
variable_sites: 8
pi: 0.0482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAACCAACGAGTCACACACT	CCCTACAAACCATTCGAGCT	45.0	50.0	57.6	57.6	303-304 bp	1.0	76.2
rpl16-rps3_p2	TGATACTACGGGGTTGGTCT	CCCTACAAACCATTCGAGCT	50.0	50.0	57.5	57.6	348-352 bp	1.0	75.4
rpl16-rps3_p3	AAACCAACGAGTCACACACT	GGGTTCCCCTACAAACCATT	45.0	50.0	57.6	57.4	309-310 bp	1.0	74.5
rpl16-rps3_p4	AAACCAACGAGTCACACACT	CCCCTACAAACCATTCGAGC	45.0	55.0	57.6	58.6	304-305 bp	1.0	74.5
rpl16-rps3_p5	TGATACTACGGGGTTGGTCT	GGGTTCCCCTACAAACCATT	50.0	50.0	57.5	57.4	354-358 bp	1.0	73.7