Hotspot

accD-psaI

LSC · 60118-60728 · 611 bp

Back to Yamazakia

Pi 0.0177

MarkerSeek score 70.40

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 611
variable_sites: 10
pi: 0.0177
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCAGAAGGGCTTATTCGACC	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.0	58.0	759-793 bp	1.0	84.5
accD-psaI_p2	CCAGAAGGGCTTATTCGACC	AAGAAGCCATTGCAATTGCC	55.0	45.0	58.0	57.9	765-799 bp	1.0	83.8
accD-psaI_p3	CCAGAAGGGCTTATTCGACC	GCCGGAAATACTAGGCCTAC	55.0	55.0	58.0	57.5	748-782 bp	1.0	81.1
accD-psaI_p4	GACCTAATCGTACCGCGTAA	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.2	58.0	743-777 bp	1.0	78.8
accD-psaI_p5	GACCTAATCGTACCGCGTAA	AAGAAGCCATTGCAATTGCC	50.0	45.0	57.2	57.9	749-783 bp	1.0	78.2