Hotspot

ndhC-trnV-UAC

LSC · 51509-52678 · 1170 bp

Back to Yamazakia

Pi 0.0351
MarkerSeek score 70.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1170
variable_sites
40
pi
0.0351
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 AATCGGGGCTAAAATACCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1296-1305 bp 1.0 80.5
ndhC-trnV-UAC_p2 GGGGCTAAAATACCGGCAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 1292-1301 bp 1.0 80.3
ndhC-trnV-UAC_p3 TTCTCCGGCCCTTTCCTAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 1313-1322 bp 1.0 79.3
ndhC-trnV-UAC_p4 CCCTTTCCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.6 57.8 1305-1314 bp 1.0 78.1
ndhC-trnV-UAC_p5 AATCGGGGCTAAAATACCGG GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.7 57.5 1295-1304 bp 1.0 77.8