Hotspot

trnY-GUA-trnE-UUC

LSC · 31692-31750 · 59 bp

Back to Yamazakia

Pi 0.0508
MarkerSeek score 70.10
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
3
pi
0.0508
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 CGAATTTACAGTCCGTCCCC CTCTCTTTCAAGGAGGCAGC 55.0 55.0 58.4 58.3 147 bp 1.0 76.7
trnY-GUA-trnE-UUC_p2 ACGAATTTACAGTCCGTCCC CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.6 58.3 148 bp 1.0 76.5
trnY-GUA-trnE-UUC_p3 GCCAACGAATTTACAGTCCG CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.5 58.3 152 bp 1.0 75.3
trnY-GUA-trnE-UUC_p4 CGAATTTACAGTCCGTCCCC GATAGTATGAGGGCGGTTGG 55.0 55.0 58.4 57.5 203 bp 1.0 74.7
trnY-GUA-trnE-UUC_p5 ACGAATTTACAGTCCGTCCC GATAGTATGAGGGCGGTTGG 50.0 55.0 57.6 57.5 204 bp 1.0 74.5