Hotspot

rps16-trnQ-UUG

LSC · 6236-7479 · 1244 bp

Back to Yamazakia

Pi 0.0449

MarkerSeek score 88.60

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1244
variable_sites: 53
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 88.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAAATTGAAATGGGGCGTGG	45.0	50.0	58.1	58.0	1338-1375 bp	1.0	85.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1280-1317 bp	1.0	85.5
rps16-trnQ-UUG_p3	GGATCGTGTCCTTCAAGTCG	GAAATTGAAATGGGGCGTGG	55.0	50.0	58.4	58.0	1352-1389 bp	1.0	83.8
rps16-trnQ-UUG_p4	GGATCGTGTCCTTCAAGTCG	GAGGTTCGAATCCTTCCGTC	55.0	55.0	58.4	58.1	1294-1331 bp	1.0	83.6
rps16-trnQ-UUG_p5	CGTTGCTTTCTACCACATCG	GAAATTGAAATGGGGCGTGG	50.0	50.0	57.2	58.0	1330-1367 bp	1.0	80.6