Hotspot

trnK-UUU-rps16

LSC · 4180-5126 · 947 bp

Back to Yamazakia

Pi 0.0407

MarkerSeek score 80.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 947
variable_sites: 37
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	1050-1066 bp	1.0	80.6
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.2	57.5	1049-1065 bp	1.0	80.6
trnK-UUU-rps16_p3	TCTAGCCGCACTTAAAAGCC	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.3	57.5	1064-1080 bp	1.0	79.8
trnK-UUU-rps16_p4	CCGCACTTAAAAGCCGAGTA	TTGAAAAAGGGGCTCAACCT	50.0	45.0	58.3	57.5	1059-1075 bp	1.0	79.7
trnK-UUU-rps16_p5	AAGCCGAGTACTCTACCGTT	AAAAGGGGCTCAACCTACAG	50.0	50.0	58.2	57.4	1045-1061 bp	1.0	79.7