Hotspot

trnH-GUG-psbA

LSC · 85-320 · 236 bp

Back to Yamazakia

Pi 0.073

MarkerSeek score 70.70

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 17
pi: 0.073
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TCAGTGCTATGCATGGTTCC	55.0	50.0	58.1	58.0	685-1016 bp	1.0	80.7
trnH-GUG-psbA_p2	GATCCACTTGGCTACATCCG	CAGTGCTATGCATGGTTCCT	55.0	50.0	58.1	58.0	684-1015 bp	1.0	80.7
trnH-GUG-psbA_p3	CAATCCACTGCCTTGATCCA	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	699-1030 bp	1.0	80.6
trnH-GUG-psbA_p4	AATCCACTGCCTTGATCCAC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	698-1029 bp	1.0	80.6
trnH-GUG-psbA_p5	ACAATCCACTGCCTTGATCC	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	700-1031 bp	1.0	80.6