Hotspot

accD

LSC · 65404-67575 · 2172 bp

Back to Xyris

Pi 0.24
MarkerSeek score 79.90
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
LSC
length_bp
2172
variable_sites
453
pi
0.24
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ATTTACGGCTCACATCGCTT TGGGTGGTTGTAGAACAGAA 45.0 45.0 58.0 56.3 2863 bp 0.5 46.2
accD_p2 GCTCACATCGCTTCATACCA TGGGTGGTTGTAGAACAGAA 50.0 45.0 58.1 56.3 2856 bp 0.5 45.9
accD_p3 TTCGCTCATTCGCCGATTTA TGGGTGGTTGTAGAACAGAA 45.0 45.0 58.1 56.3 2878 bp 0.5 45.8
accD_p4 ATTTACGGCTCACATCGCTT TGGGTGGTTGTAGAACAGAAA 45.0 42.9 58.0 57.1 2863 bp 0.5 44.3
accD_p5 ATTTACGGCTCACATCGCTT ATGGGTGGTTGTAGAACAGA 45.0 45.0 58.0 56.1 2864 bp 0.5 44.3