Hotspot

clpP-psbB

LSC · 76349-77590 · 1242 bp

Back to Xyris

Pi 0.226
MarkerSeek score 84.10
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
1242
variable_sites
267
pi
0.226
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CAAACTGCATCCTCCTCTCC ACACCCATATTCCAGGACCA 55.0 50.0 58.0 58.0 1806-1930 bp 1.0 90.0
clpP-psbB_p2 TCCTCTCCAGGAAGCTGAAA TAGCTGCCAAGAAGCACAAA 50.0 45.0 58.0 58.0 1623 bp 0.5 59.6
clpP-psbB_p3 TCCTCTCCAGGAAGCTGAAA ACACCCATATTCCAGGACCA 50.0 50.0 58.0 58.0 1794 bp 0.5 59.6
clpP-psbB_p4 CAAACTGCATCCTCCTCTCC TAGCTGCCAAGAAGCACAAA 55.0 45.0 58.0 58.0 1635 bp 0.5 59.6
clpP-psbB_p5 CCAAACTGCATCCTCCTCTC TAGCTGCCAAGAAGCACAAA 55.0 45.0 58.0 58.0 1636 bp 0.5 59.6