Hotspot

trnT-UGU-petL

LSC · 69746-71061 · 1316 bp

Back to Xyris

Pi 0.182
MarkerSeek score 80.70
Primer available yes
Label trnT-UGU-petL

Feature Metadata

label_name
trnT-UGU-petL
feature_type
igs
region
LSC
length_bp
1316
variable_sites
185
pi
0.182
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.77
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-UGU-petL_p1 TCTACCTCTGAGCTAAGCGG ACCGAAATAACTAGTTAGAGTAAGCA 55.0 34.6 58.3 57.9 1173-1365 bp 1.0 52.0
trnT-UGU-petL_p2 TCTACCTCTGAGCTAAGCGG CCGAAATAACTAGTTAGAGTAAGCA 55.0 36.0 58.3 56.5 1172-1364 bp 1.0 52.0
trnT-UGU-petL_p3 TCTACCTCTGAGCTAAGCGG ACCGAAATAACTAGTTAGAGTAAGC 55.0 36.0 58.3 56.5 1173-1365 bp 1.0 52.0
trnT-UGU-petL_p4 CTCTACCTCTGAGCTAAGCG ACCGAAATAACTAGTTAGAGTAAGCA 55.0 34.6 56.9 57.9 1174-1366 bp 1.0 52.0
trnT-UGU-petL_p5 TCTACCTCTGAGCTAAGCGG AACCGAAATAACTAGTTAGAGTAAGC 55.0 34.6 58.3 57.1 1174-1366 bp 1.0 52.0