Hotspot

psaI-trnV-UAC

LSC · 50423-51291 · 869 bp

Back to Xyris

Pi 0.114
MarkerSeek score 84.40
Primer available yes
Label psaI-trnV-UAC

Feature Metadata

label_name
psaI-trnV-UAC
feature_type
igs
region
LSC
length_bp
869
variable_sites
85
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.86
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-trnV-UAC_p1 ACTAAAGGTACAAAAATAGAGGGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 55.4 57.8 850-943 bp 1.0 51.6
psaI-trnV-UAC_p2 AAAGGTACAAAAATAGAGGGT AGAAGGTCTACGGTTCGAGT 33.3 50.0 52.3 57.8 847-940 bp 1.0 51.6
psaI-trnV-UAC_p3 ACTAAAGGTACAAAAATAGAGGGT GAAGGTCTACGGTTCGAGTC 33.3 55.0 55.4 57.5 849-942 bp 1.0 51.6
psaI-trnV-UAC_p4 CCTACTAAAGGTACAAAAATAGAGGG AGAAGGTCTACGGTTCGAGT 38.5 50.0 57.0 57.8 853-946 bp 1.0 51.6
psaI-trnV-UAC_p5 ACTAAAGGTACAAAAATAGAGGGT AGCCCAATGCAACAAAATGC 33.3 45.0 55.4 58.5 932-1025 bp 1.0 50.9