Hotspot

psbD-psbE

LSC · 42754-45193 · 2440 bp

Back to Xyris

Pi 0.128
MarkerSeek score 82.00
Primer available yes
Label psbD-psbE

Feature Metadata

label_name
psbD-psbE
feature_type
igs
region
LSC
length_bp
2440
variable_sites
257
pi
0.128
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-psbE_p1 TCTACCAAGGGCTATAGTCA CAACCCGCAATGAATAGGGA 45.0 50.0 54.3 57.9 2185-2565 bp 1.0 62.2
psbD-psbE_p2 TCTACCAAGGGCTATAGTCA ACTGACGAATAACCAACCCG 45.0 50.0 54.3 57.9 2198-2578 bp 1.0 61.9
psbD-psbE_p3 TCTACCAAGGGCTATAGTCA ACGAATAACCAACCCGCAAT 45.0 45.0 54.3 58.2 2194-2574 bp 1.0 61.2
psbD-psbE_p4 TCTACCAAGGGCTATAGTCA CGAATAACCAACCCGCAATG 45.0 50.0 54.3 57.8 2193-2573 bp 1.0 61.1
psbD-psbE_p5 TCTACCAAGGGCTATAGTCA ACCAACCCGCAATGAATAGG 45.0 50.0 54.3 58.2 2187-2567 bp 1.0 60.7