Hotspot

ycf3-trnS-GGA

LSC · 39245-39885 · 641 bp

Back to Xyris

Pi 0.0777
MarkerSeek score 85.30
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
641
variable_sites
49
pi
0.0777
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT CTACACCTTCAACCACTCGG 50.0 55.0 58.1 57.9 766-771 bp 1.0 85.0
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT GCAGTTCCAATGCTACACCT 50.0 50.0 58.1 58.2 778-783 bp 1.0 84.2
ycf3-trnS-GGA_p3 TTCTCCTGAGGTTGTCGGAA CTACACCTTCAACCACTCGG 50.0 55.0 58.3 57.9 767-772 bp 1.0 83.2
ycf3-trnS-GGA_p4 TTTCTCCTGAGGTTGTCGGA CTACACCTTCAACCACTCGG 50.0 55.0 58.3 57.9 768-773 bp 1.0 83.2
ycf3-trnS-GGA_p5 TCTCCTGAGGTTGTCGGAAT AGCAAAAGGAGAGAGAGGGA 50.0 50.0 58.1 57.7 829-834 bp 1.0 82.5