Hotspot

trnC-GCA-rpoB

LSC · 15594-16932 · 1339 bp

Back to Xyris

Pi 0.113
MarkerSeek score 89.00
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1339
variable_sites
145
pi
0.113
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
89.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG CCTCATTCTCATTCCGGAGC 50.0 55.0 58.1 58.1 1404-1414 bp 1.0 89.5
trnC-GCA-rpoB_p2 TTAGGCGACACCCAGATTTG CCTCATTCTCATTCCGGAGC 50.0 55.0 57.9 58.1 1434-1444 bp 1.0 89.1
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG TCCCTCATTCTCATTCCGGA 50.0 50.0 58.1 57.8 1406-1416 bp 1.0 89.1
trnC-GCA-rpoB_p4 TTAGGCGACACCCAGATTTG TCCCTCATTCTCATTCCGGA 50.0 50.0 57.9 57.8 1436-1446 bp 1.0 88.7
trnC-GCA-rpoB_p5 TTGTTGATTAGGCGACACCC CCTCATTCTCATTCCGGAGC 50.0 55.0 58.2 58.1 1441-1451 bp 1.0 88.5