Hotspot

psbK-psbI

LSC · 5953-6489 · 537 bp

Back to Xyris

Pi 0.0927
MarkerSeek score 84.60
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
537
variable_sites
46
pi
0.0927
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CGTGGATGTTATGCCTGTCA TCACTACTGTGTAAACAAAAAGT 50.0 30.4 58.0 54.4 644-653 bp 1.0 49.7
psbK-psbI_p2 CGTGGATGTTATGCCTGTCA TCACTACTGTGTAAACAAAAAGTT 50.0 29.2 58.0 55.1 644-653 bp 1.0 49.7
psbK-psbI_p3 TCGTGGATGTTATGCCTGTC TCACTACTGTGTAAACAAAAAGT 50.0 30.4 57.7 54.4 645-654 bp 1.0 49.7
psbK-psbI_p4 CGTGGATGTTATGCCTGTCA ATCACTACTGTGTAAACAAAAAGT 50.0 29.2 58.0 54.9 645-654 bp 1.0 49.7
psbK-psbI_p5 CGTGGATGTTATGCCTGTCA CACTACTGTGTAAACAAAAAGT 50.0 31.8 58.0 52.9 643-652 bp 1.0 49.7