Hotspot

trnK-UUU-trnQ-UUG

LSC · 4358-5320 · 963 bp

Back to Xyris

Pi 0.157
MarkerSeek score 87.90
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
963
variable_sites
136
pi
0.157
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
87.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 AGTCGCACTTAAAAGCCGAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.6 58.1 1032-1089 bp 1.0 86.8
trnK-UUU-trnQ-UUG_p2 TAGTCGCACTTAAAAGCCGA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 1033-1090 bp 1.0 85.6
trnK-UUU-trnQ-UUG_p3 TCGCACTTAAAAGCCGAGTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.3 58.1 1030-1087 bp 1.0 85.6
trnK-UUU-trnQ-UUG_p4 CGCACTTAAAAGCCGAGTAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1029-1086 bp 1.0 85.1
trnK-UUU-trnQ-UUG_p5 GTCGCACTTAAAAGCCGAGT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.9 58.1 1031-1088 bp 1.0 84.6