primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	trnK-UUU-trnQ-UUG	1	AGTCGCACTTAAAAGCCGAG	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.6	58.1	6.4	16.9	0.646638	4358	5320	1032-1089	1.000000	86.8
trnK-UUU-trnQ-UUG_p2	trnK-UUU-trnQ-UUG	2	TAGTCGCACTTAAAAGCCGA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4358	5320	1033-1090	1.000000	85.6
trnK-UUU-trnQ-UUG_p3	trnK-UUU-trnQ-UUG	3	TCGCACTTAAAAGCCGAGTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	57.3	58.1	3.0	16.9	0.804791	4358	5320	1030-1087	1.000000	85.6
trnK-UUU-trnQ-UUG_p4	trnK-UUU-trnQ-UUG	4	CGCACTTAAAAGCCGAGTAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	57.2	58.1	0.0	16.9	0.858299	4358	5320	1029-1086	1.000000	85.1
trnK-UUU-trnQ-UUG_p5	trnK-UUU-trnQ-UUG	5	GTCGCACTTAAAAGCCGAGT	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.9	58.1	6.4	16.9	0.930298	4358	5320	1031-1088	1.000000	84.6
psbK-psbI_p1	psbK-psbI	1	CGTGGATGTTATGCCTGTCA	TCACTACTGTGTAAACAAAAAGT	20	23	50.0	30.4	58.0	54.4	0.0	0.0	6.640570	5953	6489	644-653	1.000000	49.7
psbK-psbI_p2	psbK-psbI	2	CGTGGATGTTATGCCTGTCA	TCACTACTGTGTAAACAAAAAGTT	20	24	50.0	29.2	58.0	55.1	0.0	0.0	6.895991	5953	6489	644-653	1.000000	49.7
psbK-psbI_p3	psbK-psbI	3	TCGTGGATGTTATGCCTGTC	TCACTACTGTGTAAACAAAAAGT	20	23	50.0	30.4	57.7	54.4	0.0	0.0	6.927333	5953	6489	645-654	1.000000	49.7
psbK-psbI_p4	psbK-psbI	4	CGTGGATGTTATGCCTGTCA	ATCACTACTGTGTAAACAAAAAGT	20	24	50.0	29.2	58.0	54.9	0.0	0.0	7.094405	5953	6489	645-654	1.000000	49.7
psbK-psbI_p5	psbK-psbI	5	CGTGGATGTTATGCCTGTCA	CACTACTGTGTAAACAAAAAGT	20	22	50.0	31.8	58.0	52.9	0.0	0.0	7.143450	5953	6489	643-652	1.000000	49.7
petN-trnC-GCA_p1	petN-trnC-GCA	1	ACTACCATTAGAGCAGCCCA	CTGCAAATCCTTTACCCCCA	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.350555	15055	15522	550-556	1.000000	87.3
petN-trnC-GCA_p2	petN-trnC-GCA	2	ACTACCATTAGAGCAGCCCA	TGCAAATCCTTTACCCCCAG	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.350555	15055	15522	549-555	1.000000	87.3
petN-trnC-GCA_p3	petN-trnC-GCA	3	ACTACCATTAGAGCAGCCCA	ACTGCAAATCCTTTACCCCC	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.352141	15055	15522	551-557	1.000000	87.3
petN-trnC-GCA_p4	petN-trnC-GCA	4	ACTACCATTAGAGCAGCCCA	GCAAATCCTTTACCCCCAGT	20	20	50.0	50.0	58.1	57.8	0.0	0.0	0.352141	15055	15522	548-554	1.000000	87.3
petN-trnC-GCA_p5	petN-trnC-GCA	5	CAGCCCAAGCAAGACTTACT	TGCAAATCCTTTACCCCCAG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.421257	15055	15522	536-542	1.000000	87.0
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	AAAGGATTTGCAGTCCCCTG	CCTCATTCTCATTCCGGAGC	20	20	50.0	55.0	58.1	58.1	14.6	3.3	0.194810	15594	16932	1404-1414	1.000000	89.5
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	TTAGGCGACACCCAGATTTG	CCTCATTCTCATTCCGGAGC	20	20	50.0	55.0	57.9	58.1	0.0	3.3	0.231628	15594	16932	1434-1444	1.000000	89.1
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAGGATTTGCAGTCCCCTG	TCCCTCATTCTCATTCCGGA	20	20	50.0	50.0	58.1	57.8	14.6	0.0	0.245817	15594	16932	1406-1416	1.000000	89.1
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	TTAGGCGACACCCAGATTTG	TCCCTCATTCTCATTCCGGA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.282635	15594	16932	1436-1446	1.000000	88.7
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	TTGTTGATTAGGCGACACCC	CCTCATTCTCATTCCGGAGC	20	20	50.0	55.0	58.2	58.1	0.0	3.3	0.307604	15594	16932	1441-1451	1.000000	88.5
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCGTTTTGGCTGACTGTTTT	ATGCATGTTTGTTACCGGGT	20	20	40.0	45.0	56.4	58.1	0.0	6.3	1.686044	30578	31513	1286-1462	1.000000	75.6
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	TCGTTTTGGCTGACTGTTTT	GGGCTATCCGGACGTATACT	20	20	40.0	55.0	56.4	58.2	0.0	6.1	1.772336	30578	31513	1320-1496	1.000000	74.7
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	TCGTTTTGGCTGACTGTTTT	AGATCACAGAAAGGCCCTCT	20	20	40.0	50.0	56.4	58.0	0.0	0.0	1.639931	30578	31513	1193-1193	0.500000	46.9
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TCTGGAACTGGAAACTGGAA	AGATCACAGAAAGGCCCTCT	20	20	45.0	50.0	56.3	58.0	0.0	0.0	1.750092	30578	31513	1400-1400	0.500000	46.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TTCTGGAACTGGAAACTGGA	AGATCACAGAAAGGCCCTCT	20	20	45.0	50.0	56.3	58.0	0.0	0.0	1.750092	30578	31513	1401-1401	0.500000	46.0
ycf3-trnS-GGA_p1	ycf3-trnS-GGA	1	TCTCCTGAGGTTGTCGGAAT	CTACACCTTCAACCACTCGG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.141701	39245	39885	766-771	1.000000	85.0
ycf3-trnS-GGA_p2	ycf3-trnS-GGA	2	TCTCCTGAGGTTGTCGGAAT	GCAGTTCCAATGCTACACCT	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.235030	39245	39885	778-783	1.000000	84.2
ycf3-trnS-GGA_p3	ycf3-trnS-GGA	3	TTCTCCTGAGGTTGTCGGAA	CTACACCTTCAACCACTCGG	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.368230	39245	39885	767-772	1.000000	83.2
ycf3-trnS-GGA_p4	ycf3-trnS-GGA	4	TTTCTCCTGAGGTTGTCGGA	CTACACCTTCAACCACTCGG	20	20	50.0	55.0	58.3	57.9	0.0	0.0	0.368230	39245	39885	768-773	1.000000	83.2
ycf3-trnS-GGA_p5	ycf3-trnS-GGA	5	TCTCCTGAGGTTGTCGGAAT	AGCAAAAGGAGAGAGAGGGA	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.395421	39245	39885	829-834	1.000000	82.5
psbD-psbE_p1	psbD-psbE	1	TCTACCAAGGGCTATAGTCA	CAACCCGCAATGAATAGGGA	20	20	45.0	50.0	54.3	57.9	6.7	6.4	3.726827	42754	45193	2185-2565	1.000000	62.2
psbD-psbE_p2	psbD-psbE	2	TCTACCAAGGGCTATAGTCA	ACTGACGAATAACCAACCCG	20	20	45.0	50.0	54.3	57.9	6.7	0.0	3.764442	42754	45193	2198-2578	1.000000	61.9
psbD-psbE_p3	psbD-psbE	3	TCTACCAAGGGCTATAGTCA	ACGAATAACCAACCCGCAAT	20	20	45.0	45.0	54.3	58.2	6.7	0.0	3.846513	42754	45193	2194-2574	1.000000	61.2
psbD-psbE_p4	psbD-psbE	4	TCTACCAAGGGCTATAGTCA	CGAATAACCAACCCGCAATG	20	20	45.0	50.0	54.3	57.8	6.7	0.0	3.862843	42754	45193	2193-2573	1.000000	61.1
psbD-psbE_p5	psbD-psbE	5	TCTACCAAGGGCTATAGTCA	ACCAACCCGCAATGAATAGG	20	20	45.0	50.0	54.3	58.2	6.7	0.0	3.912785	42754	45193	2187-2567	1.000000	60.7
psaI-trnV-UAC_p1	psaI-trnV-UAC	1	ACTAAAGGTACAAAAATAGAGGGT	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	55.4	57.8	0.0	0.0	6.756734	50423	51291	850-943	1.000000	51.6
psaI-trnV-UAC_p2	psaI-trnV-UAC	2	AAAGGTACAAAAATAGAGGGT	AGAAGGTCTACGGTTCGAGT	21	20	33.3	50.0	52.3	57.8	0.0	0.0	6.899004	50423	51291	847-940	1.000000	51.6
psaI-trnV-UAC_p3	psaI-trnV-UAC	3	ACTAAAGGTACAAAAATAGAGGGT	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	55.4	57.5	0.0	0.0	7.100506	50423	51291	849-942	1.000000	51.6
psaI-trnV-UAC_p4	psaI-trnV-UAC	4	CCTACTAAAGGTACAAAAATAGAGGG	AGAAGGTCTACGGTTCGAGT	26	20	38.5	50.0	57.0	57.8	0.0	0.0	7.156989	50423	51291	853-946	1.000000	51.6
psaI-trnV-UAC_p5	psaI-trnV-UAC	5	ACTAAAGGTACAAAAATAGAGGGT	AGCCCAATGCAACAAAATGC	24	20	33.3	45.0	55.4	58.5	0.0	0.0	7.033510	50423	51291	932-1025	1.000000	50.9
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	AGAAGATGCGGGTTCGATTC	GCAGTTTGGTAGCTTGCAAG	20	20	50.0	50.0	58.1	57.9	0.0	22.4	0.114769	58100	58412	402-408	1.000000	91.1
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	GAGAAGATGCGGGTTCGATT	GCAGTTTGGTAGCTTGCAAG	20	20	50.0	50.0	58.1	57.9	0.0	22.4	0.114769	58100	58412	403-409	1.000000	91.1
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	AGAAGATGCGGGTTCGATTC	TTTCTTTCCTTTAGCGCGGA	20	20	50.0	45.0	58.1	57.8	0.0	8.8	0.231526	58100	58412	427-433	1.000000	89.9
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	GAGAAGATGCGGGTTCGATT	TTTCTTTCCTTTAGCGCGGA	20	20	50.0	45.0	58.1	57.8	0.0	8.8	0.231526	58100	58412	428-434	1.000000	89.8
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	AGGAACGAAGAAAATGCGGA	GCAGTTTGGTAGCTTGCAAG	20	20	45.0	50.0	57.8	57.9	0.0	22.4	0.235006	58100	58412	456-462	1.000000	89.1
trnE-UUC-trnT-GGU_p1	trnE-UUC-trnT-GGU	1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.175803	59189	60089	1005-1098	1.000000	89.7
trnE-UUC-trnT-GGU_p2	trnE-UUC-trnT-GGU	2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	20	20	50.0	50.0	57.7	58.0	0.0	0.0	0.287509	59189	60089	983-1076	1.000000	88.9
trnE-UUC-trnT-GGU_p3	trnE-UUC-trnT-GGU	3	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.8	58.2	0.0	0.0	0.320736	59189	60089	1002-1095	1.000000	88.5
trnE-UUC-trnT-GGU_p4	trnE-UUC-trnT-GGU	4	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	20	20	50.0	55.0	57.7	58.2	0.0	0.0	0.432442	59189	60089	980-1073	1.000000	87.8
trnE-UUC-trnT-GGU_p5	trnE-UUC-trnT-GGU	5	CGTTGCCTCCTTGAAAGAGA	TTGAACCGATGACTTACGCC	20	20	50.0	50.0	57.8	58.3	0.0	0.0	0.452638	59189	60089	1007-1100	1.000000	87.4
accD_p1	accD	1	ATTTACGGCTCACATCGCTT	TGGGTGGTTGTAGAACAGAA	20	20	45.0	45.0	58.0	56.3	0.0	5.0	1.727974	65404	67575	2863-2863	0.500000	46.2
accD_p2	accD	2	GCTCACATCGCTTCATACCA	TGGGTGGTTGTAGAACAGAA	20	20	50.0	45.0	58.1	56.3	0.0	5.0	1.763824	65404	67575	2856-2856	0.500000	45.9
accD_p3	accD	3	TTCGCTCATTCGCCGATTTA	TGGGTGGTTGTAGAACAGAA	20	20	45.0	45.0	58.1	56.3	0.0	5.0	1.774568	65404	67575	2878-2878	0.500000	45.8
accD_p4	accD	4	ATTTACGGCTCACATCGCTT	TGGGTGGTTGTAGAACAGAAA	20	21	45.0	42.9	58.0	57.1	0.0	5.0	1.959994	65404	67575	2863-2863	0.500000	44.3
accD_p5	accD	5	ATTTACGGCTCACATCGCTT	ATGGGTGGTTGTAGAACAGA	20	20	45.0	45.0	58.0	56.1	0.0	5.0	1.964063	65404	67575	2864-2864	0.500000	44.3
trnT-UGU-petL_p1	trnT-UGU-petL	1	TCTACCTCTGAGCTAAGCGG	ACCGAAATAACTAGTTAGAGTAAGCA	20	26	55.0	34.6	58.3	57.9	5.5	19.6	6.378260	69746	71061	1173-1365	1.000000	52.0
trnT-UGU-petL_p2	trnT-UGU-petL	2	TCTACCTCTGAGCTAAGCGG	CCGAAATAACTAGTTAGAGTAAGCA	20	25	55.0	36.0	58.3	56.5	5.5	19.6	6.844291	69746	71061	1172-1364	1.000000	52.0
trnT-UGU-petL_p3	trnT-UGU-petL	3	TCTACCTCTGAGCTAAGCGG	ACCGAAATAACTAGTTAGAGTAAGC	20	25	55.0	36.0	58.3	56.5	5.5	19.6	6.846216	69746	71061	1173-1365	1.000000	52.0
trnT-UGU-petL_p4	trnT-UGU-petL	4	CTCTACCTCTGAGCTAAGCG	ACCGAAATAACTAGTTAGAGTAAGCA	20	26	55.0	34.6	56.9	57.9	0.0	19.6	7.116894	69746	71061	1174-1366	1.000000	52.0
trnT-UGU-petL_p5	trnT-UGU-petL	5	TCTACCTCTGAGCTAAGCGG	AACCGAAATAACTAGTTAGAGTAAGC	20	26	55.0	34.6	58.3	57.1	5.5	19.6	7.242071	69746	71061	1174-1366	1.000000	52.0
clpP-psbB_p1	clpP-psbB	1	CAAACTGCATCCTCCTCTCC	ACACCCATATTCCAGGACCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.055721	76349	77590	1806-1930	1.000000	90.0
clpP-psbB_p2	clpP-psbB	2	TCCTCTCCAGGAAGCTGAAA	TAGCTGCCAAGAAGCACAAA	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.050173	76349	77590	1623-1623	0.500000	59.6
clpP-psbB_p3	clpP-psbB	3	TCCTCTCCAGGAAGCTGAAA	ACACCCATATTCCAGGACCA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.050218	76349	77590	1794-1794	0.500000	59.6
clpP-psbB_p4	clpP-psbB	4	CAAACTGCATCCTCCTCTCC	TAGCTGCCAAGAAGCACAAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.055675	76349	77590	1635-1635	0.500000	59.6
clpP-psbB_p5	clpP-psbB	5	CCAAACTGCATCCTCCTCTC	TAGCTGCCAAGAAGCACAAA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.055675	76349	77590	1636-1636	0.500000	59.6
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TGTGGAATAAATGAAATAAACGAGT	CCACTGAGCTACTGAGGA	25	18	28.0	55.6	55.6	54.8	0.0	0.0	12.593961	121298	122688	1444-2187	1.000000	52.0
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TGAAATAAACGAGTTTTTGATCA	CCACTGAGCTACTGAGGA	23	18	26.1	55.6	53.2	54.8	6.5	0.0	12.933497	121298	122688	1433-2176	1.000000	52.0
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	TGTGGAATAAATGAAATAAACGAGTT	CCACTGAGCTACTGAGGA	26	18	26.9	55.6	56.2	54.8	0.0	0.0	12.950288	121298	122688	1444-2187	1.000000	52.0
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TTGTGGAATAAATGAAATAAACGAGT	CCACTGAGCTACTGAGGA	26	18	26.9	55.6	56.2	54.8	0.0	0.0	12.950288	121298	122688	1445-2188	1.000000	52.0
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	CTTGTGGAATAAATGAAATAAACGAGT	CCACTGAGCTACTGAGGA	27	18	29.6	55.6	57.2	54.8	0.0	0.0	13.003157	121298	122688	1446-2189	1.000000	52.0