Hotspot

rpl2-rps19

LSC · 155988-156043 · 56 bp

Back to Xylosma

Pi 0.0893

MarkerSeek score 71.50

Primer available yes

Label rpl2-rps19

Feature Metadata

label_name: rpl2-rps19
feature_type: igs
region: LSC
length_bp: 56
variable_sites: 5
pi: 0.0893
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rps19_p1	AACAGTCGGACAAGTAGGGA	ATAATGGTAGATGCCCGGGA	50.0	50.0	58.0	58.0	440-441 bp	1.0	81.5
rpl2-rps19_p2	ACAGTCGGACAAGTAGGGAA	ATAATGGTAGATGCCCGGGA	50.0	50.0	58.0	58.0	439-440 bp	1.0	81.5
rpl2-rps19_p3	GACAAGTAGGGAATGCTGGG	ATAATGGTAGATGCCCGGGA	55.0	50.0	58.0	58.0	432-433 bp	1.0	81.2
rpl2-rps19_p4	GATGAAGGAACGGGAAGACC	ATAATGGTAGATGCCCGGGA	55.0	50.0	58.0	58.0	838-839 bp	1.0	80.5
rpl2-rps19_p5	CCAGATGAAGGAACGGGAAG	ATAATGGTAGATGCCCGGGA	55.0	50.0	58.0	58.0	841-842 bp	1.0	80.5