Hotspot

rps15-ycf1

SSC · 124502-124951 · 450 bp

Back to Xylosma

Pi 0.0255
MarkerSeek score 69.20
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
450
variable_sites
11
pi
0.0255
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
69.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACGGATCCCTTGTTTTCT ACCAATAATGCCAGTCGGTT 42.9 45.0 57.5 57.5 565-584 bp 1.0 68.1
rps15-ycf1_p2 TCAACGGATCCCTTGTTTTCT TGCCAGTCGGTTTAGTATGG 42.9 50.0 57.5 57.3 557-576 bp 1.0 66.7
rps15-ycf1_p3 TCAACGGATCCCTTGTTTTCT GCCAGTCGGTTTAGTATGGT 42.9 50.0 57.5 57.3 556-575 bp 1.0 66.7
rps15-ycf1_p4 TCAACGGATCCCTTGTTTTC ACCAATAATGCCAGTCGGTT 45.0 45.0 56.0 57.5 565-584 bp 1.0 63.8
rps15-ycf1_p5 CAACGGATCCCTTGTTTTCT ACCAATAATGCCAGTCGGTT 45.0 45.0 55.9 57.5 564-583 bp 1.0 63.7