Hotspot

ndhF-trnL-UAG

SSC · 114454-115131 · 678 bp

Back to Xylosma

Pi 0.0236
MarkerSeek score 63.90
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
678
variable_sites
15
pi
0.0236
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TCCATAAATATTGATATGTATGTTCCA AGCGTGTCTACCGATTTCAC 25.9 50.0 54.9 58.0 701-735 bp 1.0 45.7
ndhF-trnL-UAG_p2 TCCATAAATATTGATATGTATGTTCCA CAGCGTGTCTACCGATTTCA 25.9 50.0 54.9 58.0 702-736 bp 1.0 45.7
ndhF-trnL-UAG_p3 TCCATAAATATTGATATGTATGTTCCA CCTAAGAGCAGCGTGTCTAC 25.9 55.0 54.9 57.8 710-744 bp 1.0 45.7
ndhF-trnL-UAG_p4 TCCATAAATATTGATATGTATGTTCCA CTAAGAGCAGCGTGTCTACC 25.9 55.0 54.9 57.8 709-743 bp 1.0 45.7
ndhF-trnL-UAG_p5 TCCATAAATATTGATATGTATGTTCCA TCCTAAGAGCAGCGTGTCTA 25.9 50.0 54.9 57.9 711-745 bp 1.0 45.6