Hotspot

trnR-ACG-trnN-GUU

SSC · 109279-109942 · 664 bp

Back to Xylosma

Pi 0.0122

MarkerSeek score 67.20

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 664
variable_sites: 8
pi: 0.0122
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	841-854 bp	1.0	81.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	805-818 bp	1.0	80.0
trnR-ACG-trnN-GUU_p3	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	805-818 bp	1.0	80.0
trnR-ACG-trnN-GUU_p4	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	792-805 bp	1.0	79.9
trnR-ACG-trnN-GUU_p5	AACAGATCCAGTGGAGACGA	CTGGTCGTAGGTTCGAATCC	50.0	55.0	58.1	57.8	793-806 bp	1.0	79.6