Hotspot

psbT-psbN

LSC · 74435-74494 · 60 bp

Back to Xylosma

Pi 0.0333

MarkerSeek score 71.70

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 2
pi: 0.0333
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAGAACCGCCTAAAGTTC	GATCCATTCGAGGAACACGG	50.0	55.0	57.6	58.4	127 bp	1.0	75.0
psbT-psbN_p2	GAGAACCGCCTAAAGTTCCA	GATCCATTCGAGGAACACGG	50.0	55.0	57.5	58.4	125 bp	1.0	74.1
psbT-psbN_p3	TCGAGAACCGCCTAAAGTTC	GGCAACCCTCTCAACAACTA	50.0	50.0	57.6	57.4	150 bp	1.0	73.7
psbT-psbN_p4	TCGAGAACCGCCTAAAGTTC	AGATCCATTCGAGGAACACG	50.0	50.0	57.6	57.4	128 bp	1.0	73.7
psbT-psbN_p5	GAGAACCGCCTAAAGTTCCA	GGCAACCCTCTCAACAACTA	50.0	50.0	57.5	57.4	148 bp	1.0	72.8