Hotspot

petA-psbJ

LSC · 62914-63845 · 932 bp

Back to Xylosma

Pi 0.0449

MarkerSeek score 78.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 932
variable_sites: 38
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.2	57.6	991-992 bp	1.0	70.0
petA-psbJ_p2	TGAGAAGGTTCAATTGTCCGAA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	991-992 bp	1.0	67.5
petA-psbJ_p3	TTGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	40.9	50.0	57.9	57.6	992-993 bp	1.0	67.5
petA-psbJ_p4	TGAGAAGGTTCAATTGTCCGA	ATGGCTCCTATTCCGGATTG	42.9	50.0	57.2	57.1	1000-1001 bp	1.0	66.0
petA-psbJ_p5	TGAGAAGGTTCAATTGTCCG	ATTCCGGATTGGGTTCATCC	45.0	50.0	55.7	57.6	991-992 bp	1.0	65.6