Hotspot

trnS-UGA-lhbA

LSC · 35417-35755 · 339 bp

Back to Xylosma

Pi 0.0396

MarkerSeek score 73.50

Primer available yes

Label trnS-UGA-lhbA

Feature Metadata

label_name: trnS-UGA-lhbA
feature_type: igs
region: LSC
length_bp: 339
variable_sites: 13
pi: 0.0396
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-lhbA_p1	CAAGACCGGAGCTATCAACC	TTTTGTTGCTTGACCAACCG	55.0	45.0	58.1	57.7	485-551 bp	1.0	81.0
trnS-UGA-lhbA_p2	CTATCAACCACTCGGCCATC	TTTTGTTGCTTGACCAACCG	55.0	45.0	58.1	57.7	474-540 bp	1.0	80.5
trnS-UGA-lhbA_p3	CAAGACCGGAGCTATCAACC	CCAACCGTCAGGAGAAGAAA	55.0	50.0	58.1	57.5	472-538 bp	1.0	79.0
trnS-UGA-lhbA_p4	GAACCCTCGATAGTTCGCAA	TTTTGTTGCTTGACCAACCG	50.0	45.0	57.7	57.7	522-588 bp	1.0	78.9
trnS-UGA-lhbA_p5	ACCCTCGATAGTTCGCAAAG	TTTTGTTGCTTGACCAACCG	50.0	45.0	57.7	57.7	520-586 bp	1.0	78.8