Hotspot

psbI-trnS-GCU

LSC · 6924-7060 · 137 bp

Back to Xylosma

Pi 0.0299

MarkerSeek score 71.70

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 4
pi: 0.0299
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	ATGATCCAGGGCGTAATCCT	TAAAGCGCCGGATTGCTAAT	50.0	45.0	58.3	58.0	237-240 bp	1.0	79.9
psbI-trnS-GCU_p2	ATGATCCAGGGCGTAATCCT	ATTGGGAGAGATGGCTGAGT	50.0	50.0	58.3	58.1	261-264 bp	1.0	79.1
psbI-trnS-GCU_p3	AATGATCCAGGGCGTAATCC	TAAAGCGCCGGATTGCTAAT	50.0	45.0	57.4	58.0	238-241 bp	1.0	77.5
psbI-trnS-GCU_p4	ATGATCCAGGGCGTAATCCT	CGAGTTATTCGTACCGAGGG	50.0	55.0	58.3	57.6	209-212 bp	1.0	77.4
psbI-trnS-GCU_p5	ATGATCCAGGGCGTAATCCT	ATGGCTGAGTGGACTAAAGC	50.0	50.0	58.3	57.6	251-254 bp	1.0	76.8