Hotspot

rps16

LSC · 4807-5936 · 1130 bp

Back to Xylosalsola

Pi 0.00265

MarkerSeek score 59.70

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1130
variable_sites: 3
pi: 0.00265
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	CAAACTACAACACTACAACCACA	CGTGTATTGACTGTTCTGGC	39.1	50.0	57.2	56.8	1300 bp	1.0	40.4
rps16_p2	ACTACAACACTACAACCACA	CGTGTATTGACTGTTCTGGC	40.0	50.0	54.2	56.8	1297 bp	1.0	40.2
rps16_p3	CAAACTACAACACTACAACCACA	CGTGTATTGACTGTTCTGGCT	39.1	47.6	57.2	58.3	1300 bp	1.0	40.2
rps16_p4	CAAACTACAACACTACAACCACA	TCCGTGTATTGACTGTTCTGG	39.1	47.6	57.2	57.7	1302 bp	1.0	40.2
rps16_p5	AACTACAACACTACAACCACA	CGTGTATTGACTGTTCTGGC	38.1	50.0	55.0	56.8	1298 bp	1.0	40.2