Hotspot

ndhK

LSC · 49406-50089 · 684 bp

Back to Xylosalsola

Pi 0.00146

MarkerSeek score 59.60

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 684
variable_sites: 1
pi: 0.00146
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	ACCTGCTGCTTTCAATTCCA	TTTATGCATGGCGAAAGGGA	45.0	45.0	58.0	57.9	816 bp	1.0	79.0
ndhK_p2	ACCTGCTGCTTTCAATTCCA	GTTTATGCATGGCGAAAGGG	45.0	50.0	58.0	57.8	817 bp	1.0	78.4
ndhK_p3	ACCTGCTGCTTTCAATTCCA	TTATGCATGGCGAAAGGGAG	45.0	50.0	58.0	58.3	815 bp	1.0	77.5
ndhK_p4	GACCTGCTGCTTTCAATTCC	TTTATGCATGGCGAAAGGGA	50.0	45.0	57.4	57.9	817 bp	1.0	73.9
ndhK_p5	GACCTGCTGCTTTCAATTCC	GTTTATGCATGGCGAAAGGG	50.0	50.0	57.4	57.8	818 bp	1.0	73.3