Hotspot

atpI

LSC · 14098-14841 · 744 bp

Back to Xylosalsola

Pi 0.00134
MarkerSeek score 59.50
Primer available yes
Label atpI

Feature Metadata

label_name
atpI
feature_type
gene
region
LSC
length_bp
744
variable_sites
1
pi
0.00134
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpI_p1 AGACTATCTTGAGCCACACA AGGTCGAGGAAGAGATGGTT 45.0 50.0 55.9 57.8 894 bp 1.0 61.2
atpI_p2 AGACTATCTTGAGCCACACA GGTCGAGGAAGAGATGGTTG 45.0 55.0 55.9 57.7 893 bp 1.0 60.7
atpI_p3 AGACTATCTTGAGCCACACA ACAGGTCGAGGAAGAGATGG 45.0 55.0 55.9 58.5 896 bp 1.0 59.0
atpI_p4 AGACTATCTTGAGCCACACA CAGGTCGAGGAAGAGATGGT 45.0 55.0 55.9 58.5 895 bp 1.0 59.0
atpI_p5 AGACTATCTTGAGCCACACAT AGGTCGAGGAAGAGATGGTT 42.9 50.0 56.4 57.8 894 bp 1.0 57.7