Hotspot

atpF

LSC · 11370-12723 · 1354 bp

Back to Xylosalsola

Pi 0.00371

MarkerSeek score 61.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1354
variable_sites: 5
pi: 0.00371
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCTAATTTCGTCTGCTCGA	GGAATTGGGGAATCTCAATT	45.0	40.0	57.4	53.1	1568-1573 bp	1.0	40.6
atpF_p2	TGCTAATTTCGTCTGCTCGAA	GGAATTGGGGAATCTCAATT	42.9	40.0	58.0	53.1	1568-1573 bp	1.0	40.6
atpF_p3	TTGCTAATTTCGTCTGCTCGA	GGAATTGGGGAATCTCAATT	42.9	40.0	58.0	53.1	1569-1574 bp	1.0	40.6
atpF_p4	CGTCTGCTCGAATAGTTACCA	GGAATTGGGGAATCTCAATT	47.6	40.0	57.6	53.1	1559-1564 bp	1.0	40.6
atpF_p5	TTGCTAATTTCGTCTGCTCG	GGAATTGGGGAATCTCAATT	45.0	40.0	56.6	53.1	1569-1574 bp	1.0	40.6