Hotspot

ndhF-rpl32

SSC · 110293-111409 · 1117 bp

Back to Xylosalsola

Pi 0.00629

MarkerSeek score 62.80

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1117
variable_sites: 7
pi: 0.00629
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTTGTTGCCGTCGGAAAAA	AAAAGGCTTTTAACGCTGCC	40.0	45.0	57.4	57.9	1311-1316 bp	1.0	74.3
ndhF-rpl32_p2	TTTTTGTTGCCGTCGGAAAA	AAAAGGCTTTTAACGCTGCC	40.0	45.0	57.4	57.9	1312-1317 bp	1.0	74.3
ndhF-rpl32_p3	TTTTGTTGCCGTCGGAAAAA	TTTAACGCTGCCCAATACCC	40.0	50.0	57.4	58.5	1303-1308 bp	1.0	71.3
ndhF-rpl32_p4	TTTTTGTTGCCGTCGGAAAA	TTTAACGCTGCCCAATACCC	40.0	50.0	57.4	58.5	1304-1309 bp	1.0	71.3
ndhF-rpl32_p5	TTTTTGTTGCCGTCGGAAAAA	AAAAGGCTTTTAACGCTGCC	38.1	45.0	58.0	57.9	1312-1317 bp	1.0	71.2