Hotspot

trnR-ACG-trnN-GUU

IRb · 106644-107248 · 605 bp

Back to Xylosalsola

Pi 0.00165

MarkerSeek score 59.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 605
variable_sites: 1
pi: 0.00165
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	GGATACTCCCGGGAACAGAT	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.3	58.1	836 bp	1.0	77.1
trnR-ACG-trnN-GUU_p2	GGATACTCCCGGGAACAGAT	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.3	58.2	746 bp	1.0	76.6
trnR-ACG-trnN-GUU_p3	CAGTGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	58.4	58.1	787 bp	1.0	76.5
trnR-ACG-trnN-GUU_p4	GGATACTCCCGGGAACAGAT	GGGTTGAGAACGGGAATTGA	55.0	50.0	58.3	57.8	835 bp	1.0	76.3
trnR-ACG-trnN-GUU_p5	CAGTGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	58.4	58.2	697 bp	1.0	76.0