Hotspot

petA-psbJ

LSC · 62855-63841 · 987 bp

Back to Xylosalsola

Pi 0.00203
MarkerSeek score 59.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
987
variable_sites
2
pi
0.00203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACTGCGTGTACAAAGCCTTT GCCGATACTACTGGAAGGAT 45.0 50.0 58.3 56.2 1222-1226 bp 1.0 63.2
petA-psbJ_p2 ACTGCGTGTACAAAGCCTTT CTGGATTAGGCTCATCACTGT 45.0 47.6 58.3 57.2 1125-1129 bp 1.0 62.9
petA-psbJ_p3 ACTGCGTGTACAAAGCCTTT GCCGATACTACTGGAAGGATT 45.0 47.6 58.3 57.0 1222-1226 bp 1.0 61.3
petA-psbJ_p4 CGAGAAGGTTCAACTGTCCG GCCGATACTACTGGAAGGAT 55.0 50.0 58.6 56.2 1141-1145 bp 1.0 61.1
petA-psbJ_p5 ACTGCGTGTACAAAGCCTTT TGGATTAGGCTCATCACTGT 45.0 45.0 58.3 55.9 1124-1128 bp 1.0 60.9