Hotspot

trnT-UGU-trnL-UAA

LSC · 46359-47026 · 668 bp

Back to Xylosalsola

Pi 0.0015

MarkerSeek score 59.60

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 1
pi: 0.0015
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	AGCCAATTAAGTCCGTAGCG	55.0	50.0	57.9	58.1	741-756 bp	1.0	78.5
trnT-UGU-trnL-UAA_p2	GGAATCGAACCGATGACCAT	AGCCAATTAAGTCCGTAGCG	50.0	50.0	57.8	58.1	773-788 bp	1.0	77.7
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	729-744 bp	1.0	77.1
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GCCAATTAAGTCCGTAGCGT	55.0	50.0	57.9	58.4	740-755 bp	1.0	76.2
trnT-UGU-trnL-UAA_p5	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	761-776 bp	1.0	76.2