Hotspot

psaA-ycf3

LSC · 41464-42196 · 733 bp

Back to Xylosalsola

Pi 0.00273

MarkerSeek score 60.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 2
pi: 0.00273
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGAAAAATGACCGGGTCTGG	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.1	58.0	929-930 bp	1.0	79.2
psaA-ycf3_p2	TGAAAAATGACCGGGTCTGG	TTGGTTGAAGATCACAGGGC	50.0	50.0	58.1	58.1	862-863 bp	1.0	78.8
psaA-ycf3_p3	TGAAAAATGACCGGGTCTGG	CAGAGGCTTGGTTCGATCAA	50.0	50.0	58.1	57.9	944-945 bp	1.0	78.7
psaA-ycf3_p4	TGAAAAATGACCGGGTCTGG	CGATCAAGCCGCTGAGTATT	50.0	50.0	58.1	58.1	931-932 bp	1.0	78.4
psaA-ycf3_p5	TGAAAAATGACCGGGTCTGG	TGAAGATCACAGGGCGTTTC	50.0	50.0	58.1	58.2	857-858 bp	1.0	78.0