Hotspot

matK-trnK-UUU

LSC · 3442-4162 · 721 bp

Back to Xylosalsola

Pi 0.00139

MarkerSeek score 60.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 721
variable_sites: 1
pi: 0.00139
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCAAGTTATGTTGCCAAGACCT	AACGGTAGAGTACTCGGCTT	40.9	50.0	57.9	58.2	796-800 bp	1.0	62.4
matK-trnK-UUU_p2	TCAAGTTATGTTGCCAAGACCT	ACGGTAGAGTACTCGGCTTT	40.9	50.0	57.9	58.2	795-799 bp	1.0	62.4
matK-trnK-UUU_p3	TCAAGTTATGTTGCCAAGACCT	GGGTTGCTAACTCAACGGTA	40.9	50.0	57.9	57.5	809-813 bp	1.0	60.0
matK-trnK-UUU_p4	TCAAGTTATGTTGCCAAGACCT	TTCGGGTTGCTAACTCAACG	40.9	50.0	57.9	58.5	812-816 bp	1.0	59.7
matK-trnK-UUU_p5	TCAAGTTATGTTGCCAAGACC	ACGGTAGAGTACTCGGCTTT	42.9	50.0	56.4	58.2	795-799 bp	1.0	58.7