Hotspot

ycf3

LSC · 44765-46803 · 2039 bp

Back to Xylocarpus

Pi 0.00589

MarkerSeek score 59.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2039
variable_sites: 12
pi: 0.00589
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	TTTATTTCACCGATGGGGCC	TGGGATCATTTTCTCACGAGAG	50.0	45.5	58.2	57.9	2185-2223 bp	1.0	61.6
ycf3_p2	TTTATTTCACCGATGGGGCC	GGATCATTTTCTCACGAGAGGT	50.0	45.5	58.2	57.9	2183-2221 bp	1.0	61.6
ycf3_p3	TTTATTTCACCGATGGGGCC	GTGGGATCATTTTCTCACGAGA	50.0	45.5	58.2	58.1	2186-2224 bp	1.0	61.5
ycf3_p4	TTTATTTCACCGATGGGGCC	TGGGATCATTTTCTCACGAGA	50.0	42.9	58.2	56.7	2185-2223 bp	1.0	60.5
ycf3_p5	TTTATTTCACCGATGGGGCC	GTGGGATCATTTTCTCACGAG	50.0	47.6	58.2	56.7	2186-2224 bp	1.0	60.2