Hotspot

ycf1

SSC · 128038-133404 · 5367 bp

Back to Xylocarpus

Pi 0.00188

MarkerSeek score 54.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5367
variable_sites: 10
pi: 0.00188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GACCGCTTAACCAGTTTTTCC	AACGAAGCAAATCAGGAAAA	47.6	35.0	57.7	54.0	340 bp	1.0	40.5
ycf1_p2	ACCGCTTAACCAGTTTTTCCA	AACGAAGCAAATCAGGAAAA	42.9	35.0	58.3	54.0	339 bp	1.0	40.5
ycf1_p3	CCGCTTAACCAGTTTTTCCA	AACGAAGCAAATCAGGAAAA	45.0	35.0	56.5	54.0	338 bp	1.0	40.5
ycf1_p4	ACCGCTTAACCAGTTTTTCC	AACGAAGCAAATCAGGAAAA	45.0	35.0	56.5	54.0	339 bp	1.0	40.5
ycf1_p5	GACCGCTTAACCAGTTTTTCC	ACGAAGCAAATCAGGAAAAT	47.6	35.0	57.7	53.8	339 bp	1.0	40.5