Hotspot

rps3

LSC · 86258-86917 · 660 bp

Back to Xylocarpus

Pi 0

MarkerSeek score 38.90

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 660
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGGGTAGACTGTAAAGACTCGT	AGATGGAACTGTGGGGGATT	45.5	50.0	58.2	58.3	708 bp	1.0	59.6
rps3_p2	TCAACCCTATAGAATTGCGGA	AGATGGAACTGTGGGGGATT	42.9	50.0	56.5	58.3	794 bp	1.0	57.6
rps3_p3	TGGGTAGACTGTAAAGACTCG	AGATGGAACTGTGGGGGATT	47.6	50.0	56.5	58.3	708 bp	1.0	57.6
rps3_p4	GGGTAGACTGTAAAGACTCGT	AGATGGAACTGTGGGGGATT	47.6	50.0	56.5	58.3	707 bp	1.0	57.6
rps3_p5	TGGGTAGACTGTAAAGACTCGT	GAGATGGAACTGTGGGGGAT	45.5	55.0	58.2	58.8	709 bp	1.0	55.8