Hotspot

rpl32-trnL-UAG

SSC · 117693-118468 · 776 bp

Back to Xylocarpus

Pi 0.00516
MarkerSeek score 61.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
776
variable_sites
4
pi
0.00516
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 CGAAATCCCTTTCTACCGGG AGCGTGTCTACCGATTTCAC 55.0 50.0 58.1 58.0 860-861 bp 1.0 80.0
rpl32-trnL-UAG_p2 CGAAATCCCTTTCTACCGGG CAGCGTGTCTACCGATTTCA 55.0 50.0 58.1 58.0 861-862 bp 1.0 80.0
rpl32-trnL-UAG_p3 CGAAATCCCTTTCTACCGGG TCCTAAGAGCAGCGTGTCTA 55.0 50.0 58.1 57.9 870-871 bp 1.0 79.1
rpl32-trnL-UAG_p4 CGAAATCCCTTTCTACCGGG CCTAAGAGCAGCGTGTCTAC 55.0 55.0 58.1 57.8 869-870 bp 1.0 78.5
rpl32-trnL-UAG_p5 CGAAATCCCTTTCTACCGGG CTAAGAGCAGCGTGTCTACC 55.0 55.0 58.1 57.8 868-869 bp 1.0 78.5