Hotspot

trnG-UCC-trnR-UCU

LSC · 10802-10956 · 155 bp

Back to Xylocarpus

Pi 0.0129

MarkerSeek score 59.90

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 155
variable_sites: 2
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAAGAAATTGCGTCCAA	50.0	40.0	58.0	56.2	282 bp	1.0	65.6
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAAGAAATTGCGTCCAA	55.0	40.0	58.1	56.2	285 bp	1.0	65.3
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	235 bp	1.0	64.0
trnG-UCC-trnR-UCU_p4	AGCCTTCCAAGCTAACGATG	GGGAAATGGAAAGAAATTGCGT	50.0	40.9	58.0	58.1	288 bp	1.0	63.7
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	ATGGAAAGAAATTGCGTCCA	50.0	40.0	58.0	55.9	283 bp	1.0	63.7