Hotspot

trnS-GCU-trnG-UCC

LSC · 9208-10001 · 794 bp

Back to Xylocarpus

Pi 0.00254
MarkerSeek score 61.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
794
variable_sites
2
pi
0.00254
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TAAGCCACTCAGCCATCTCT AACTGAAGGACCCCTTAACT 50.0 45.0 58.1 55.6 880-888 bp 1.0 61.0
trnS-GCU-trnG-UCC_p2 TAAGCCACTCAGCCATCTCT ACTTAAACTGAAGGACCCCT 50.0 45.0 58.1 55.6 885-893 bp 1.0 61.0
trnS-GCU-trnG-UCC_p3 ACGGAAAGAGAGGGATTCGA AACTGAAGGACCCCTTAACT 50.0 45.0 58.2 55.6 947-955 bp 1.0 60.8
trnS-GCU-trnG-UCC_p4 ACGGAAAGAGAGGGATTCGA ACTTAAACTGAAGGACCCCT 50.0 45.0 58.2 55.6 952-960 bp 1.0 60.8
trnS-GCU-trnG-UCC_p5 AAGATACCAAGCCATCGACG AACTGAAGGACCCCTTAACT 50.0 45.0 57.8 55.6 970-978 bp 1.0 60.2