Hotspot

matK-trnK-UUU

LSC · 3714-4432 · 719 bp

Back to Xylocarpus

Pi 0.00278

MarkerSeek score 60.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 719
variable_sites: 2
pi: 0.00278
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACTCCCGAAAAAGAAGTGGG	CGTTGTGCAATTCAATCGCT	50.0	45.0	57.7	58.0	900-901 bp	1.0	77.7
matK-trnK-UUU_p2	CTCCCGAAAAAGAAGTGGGT	CGTTGTGCAATTCAATCGCT	50.0	45.0	57.7	58.0	899-900 bp	1.0	77.7
matK-trnK-UUU_p3	ACTCCCGAAAAAGAAGTGGG	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	821-822 bp	1.0	76.7
matK-trnK-UUU_p4	CTCCCGAAAAAGAAGTGGGT	AACGGTAGAGTACTCGGCTT	50.0	50.0	57.7	58.2	821-822 bp	1.0	76.7
matK-trnK-UUU_p5	CTCCCGAAAAAGAAGTGGGT	ACGGTAGAGTACTCGGCTTT	50.0	50.0	57.7	58.2	820-821 bp	1.0	76.7