Hotspot

ycf1

IRb · 114312-119804 · 5493 bp

Pi 0.0134

MarkerSeek score 46.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 5493
variable_sites: 73
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGGGACGATGCTTATACGGA	GGAACGCCATTATAGGTGCA	50.0	50.0	58.0	58.0	614-620 bp	1.0	83.5
ycf1_p2	AGGGACGATGCTTATACGGA	TGGAACGCCATTATAGGTGC	50.0	50.0	58.0	58.0	615-621 bp	1.0	83.5
ycf1_p3	AGGGACGATGCTTATACGGA	TCTGTGCCAAGGTTTCAGAC	50.0	50.0	58.0	58.0	705-711 bp	1.0	83.2
ycf1_p4	AGGGACGATGCTTATACGGA	TGCCAAGGTTTCAGACGAAA	50.0	45.0	58.0	58.0	701-707 bp	1.0	83.1
ycf1_p5	AGGGACGATGCTTATACGGA	CGTTTAGCCAGTCTTTCGGA	50.0	50.0	58.0	57.9	652-658 bp	1.0	83.1