Hotspot

ndhG-ndhI

IRa · 139108-139726 · 619 bp

Pi 0.0217

MarkerSeek score 74.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: IRa
length_bp: 619
variable_sites: 13
pi: 0.0217
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGACCCGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	55.0	45.0	58.0	57.9	733-752 bp	1.0	82.4
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	732-751 bp	1.0	82.3
ndhG-ndhI_p3	TCCCAACGAAAAGGCAGAAA	AATCAAATTGCTTTGGGCCG	45.0	45.0	57.9	57.9	795-814 bp	1.0	81.9
ndhG-ndhI_p4	AGACCCGATCCCAGAAAGAT	AATCAAATTGCTTTGGGCCG	50.0	45.0	57.8	57.9	731-750 bp	1.0	81.2
ndhG-ndhI_p5	CAAGACCCGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	55.0	45.0	58.0	58.3	722-741 bp	1.0	80.9