Hotspot

ndhF-rpl32

SSC · 132807-133717 · 911 bp

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Pi 0.0432
MarkerSeek score 69.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
911
variable_sites
39
pi
0.0432
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GCAGCCCCACTCCTATTAAC AGCGTGTCTACCGATTTCAC 55.0 50.0 58.0 58.0 1617-1674 bp 1.0 86.8
ndhF-rpl32_p2 GCAGCCCCACTCCTATTAAC CAGCGTGTCTACCGATTTCA 55.0 50.0 58.0 58.0 1618-1675 bp 1.0 86.8
ndhF-rpl32_p3 TAGGGCGTGTGAACCAAAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 2046-2103 bp 1.0 85.4
ndhF-rpl32_p4 TAGGGCGTGTGAACCAAAAA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 2047-2104 bp 1.0 85.4
ndhF-rpl32_p5 AGCAACTGAACCGCCAAATA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2255-2312 bp 1.0 84.6