Hotspot

trnL-UAG-ndhF

IRb · 130129-130559 · 431 bp

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Pi 0.0932
MarkerSeek score 79.00
Primer available yes
Label trnL-UAG-ndhF

Feature Metadata

label_name
trnL-UAG-ndhF
feature_type
igs
region
IRb
length_bp
431
variable_sites
37
pi
0.0932
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.92
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ndhF_p1 AGCGTGTCTACCGATTTCAC AAAATATGCAGGAGGTGGCC 50.0 50.0 58.0 58.2 566 bp 0.5 58.2
trnL-UAG-ndhF_p2 CAGCGTGTCTACCGATTTCA AAAATATGCAGGAGGTGGCC 50.0 50.0 58.0 58.2 567 bp 0.5 58.2
trnL-UAG-ndhF_p3 TATCATGCCCTTTTCGGACG AAAATATGCAGGAGGTGGCC 50.0 50.0 58.1 58.2 853 bp 0.5 57.8
trnL-UAG-ndhF_p4 GCTGCATAGCCCAATACCAT AAAATATGCAGGAGGTGGCC 50.0 50.0 58.1 58.2 1167 bp 0.5 57.6
trnL-UAG-ndhF_p5 GAAGTAGCAAAGCCTTGGGT AAAATATGCAGGAGGTGGCC 50.0 50.0 58.1 58.2 1065 bp 0.5 57.5