Hotspot

rps11-rpl36

LSC · 83619-83913 · 295 bp

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Pi 0.0272
MarkerSeek score 53.90
Primer available yes
Label rps11-rpl36

Feature Metadata

label_name
rps11-rpl36
feature_type
igs
region
LSC
length_bp
295
variable_sites
8
pi
0.0272
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11-rpl36_p1 TACGCGCATTCTTACGTGAA ACCCCGGAACAAAAATTCGA 45.0 45.0 58.0 58.0 644-671 bp 1.0 82.8
rps11-rpl36_p2 TTTTAGGGGGCCTACATCCA ACCCCGGAACAAAAATTCGA 50.0 45.0 58.0 58.0 977-1004 bp 1.0 82.4
rps11-rpl36_p3 ACTTCCACTGTAGTGTCCGA ACCCCGGAACAAAAATTCGA 50.0 45.0 58.0 58.0 1117-1144 bp 1.0 82.3
rps11-rpl36_p4 TTCCACTGTAGTGTCCGAGT ACCCCGGAACAAAAATTCGA 50.0 45.0 58.0 58.0 1115-1142 bp 1.0 82.3
rps11-rpl36_p5 GAAATCACCTGACCCCGTAC ACCCCGGAACAAAAATTCGA 55.0 45.0 58.0 58.0 729 bp 0.5 59.7