Hotspot

psbJ-clpP

LSC · 74253-75163 · 911 bp

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Pi 0.073
MarkerSeek score 77.40
Primer available yes
Label psbJ-clpP

Feature Metadata

label_name
psbJ-clpP
feature_type
igs
region
LSC
length_bp
911
variable_sites
65
pi
0.073
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbJ-clpP_p1 AACTGGCCGTTTTGATCCTT TATCGACTCAACTTGCTGGC 45.0 50.0 58.0 58.0 2051 bp 0.5 59.9
psbJ-clpP_p2 ACTGGCCGTTTTGATCCTTT TATCGACTCAACTTGCTGGC 45.0 50.0 58.0 58.0 2050 bp 0.5 59.9
psbJ-clpP_p3 AACTGGCCGTTTTGATCCTT CCTGGGGAAGAAGATGCATC 45.0 55.0 58.0 58.0 2142 bp 0.5 59.8
psbJ-clpP_p4 ACTGGCCGTTTTGATCCTTT CCTGGGGAAGAAGATGCATC 45.0 55.0 58.0 58.0 2141 bp 0.5 59.8
psbJ-clpP_p5 ACTGGCCGTTTTGATCCTTT TGCTGTATCCGAACACATGG 45.0 50.0 58.0 58.0 1677 bp 0.5 59.8