Hotspot

rbcL-accD

LSC · 60618-61596 · 979 bp

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Pi 0.0193
MarkerSeek score 74.40
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
979
variable_sites
18
pi
0.0193
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 AGTCCTGAATTAGCTGCTGC TGGGTCTTCACTTACACTGG 50.0 50.0 58.0 56.8 1135-1174 bp 1.0 74.4
rbcL-accD_p2 AGTCCTGAATTAGCTGCTGC GGGTCTTCACTTACACTGGT 50.0 50.0 58.0 56.8 1134-1173 bp 1.0 74.4
rbcL-accD_p3 GAGGCTAGCAAATGGAGTCC TGGGTCTTCACTTACACTGG 55.0 50.0 58.0 56.8 1150-1189 bp 1.0 74.4
rbcL-accD_p4 GAGGCTAGCAAATGGAGTCC GGGTCTTCACTTACACTGGT 55.0 50.0 58.0 56.8 1149-1188 bp 1.0 74.3
rbcL-accD_p5 TTCGTGAGGCTAGCAAATGG TGGGTCTTCACTTACACTGG 50.0 50.0 58.3 56.8 1155-1194 bp 1.0 72.6